Journal: Oncotarget

Article Title: Preliminary safety and imaging efficacy of the near-infrared fluorescent contrast agent DA364 during fluorescence-guided surgery in dogs with spontaneous superficial tumors

doi: 10.18632/oncotarget.27633

Figure Lengend Snippet: ( A ) Representative sigmoid curve revealing single-digit nanomolar affinity of DA364 for the human α v β 3 integrin receptor. ( B ) Dose-response inhibition of DA364 uptake in human melanoma WM266 cells by the unconjugated cRGD vector.

Article Snippet: A 96-well microtiter plate was coated overnight at 4° C with 1 μg/mL recombinant human α v β 3 integrin (Merck-Millipore, Darmstadt, Germany) in coating buffer (20 mM Tris HCl pH 7.4; 150 mM NaCl; 1 mM MnCl 2 ; 0.5 mM MgCl 2 ; 2 mM CaCl 2 ).

Techniques: Inhibition, Plasmid Preparation

Journal: Oncotarget

Article Title: Preliminary safety and imaging efficacy of the near-infrared fluorescent contrast agent DA364 during fluorescence-guided surgery in dogs with spontaneous superficial tumors

doi: 10.18632/oncotarget.27633

Figure Lengend Snippet: The workflow consists of the intravenous administration of DA364 24 h or 48 h prior to surgery ( A ), followed by pre-operative imaging ( B , C : procedure and fluorescent image in panel respectively), intra-operative imaging ( D , E : procedure and correspondent fluorescent image in panel respectively) and post-operative imaging ( F , G : procedure and correspondent fluorescent image, visualized on screen, respectively). Excised tissues were sliced in 4 μm-thick sections and underwent tumor assessment by Hematoxylin and Eosin histopathology ( H ), and evaluation of the β 3 integrin by immunohistochemistry ( I ).

Article Snippet: A 96-well microtiter plate was coated overnight at 4° C with 1 μg/mL recombinant human α v β 3 integrin (Merck-Millipore, Darmstadt, Germany) in coating buffer (20 mM Tris HCl pH 7.4; 150 mM NaCl; 1 mM MnCl 2 ; 0.5 mM MgCl 2 ; 2 mM CaCl 2 ).

Techniques: Imaging, Histopathology, Immunohistochemistry

Journal: Oncotarget

Article Title: Preliminary safety and imaging efficacy of the near-infrared fluorescent contrast agent DA364 during fluorescence-guided surgery in dogs with spontaneous superficial tumors

doi: 10.18632/oncotarget.27633

Figure Lengend Snippet: ( A ) Western blot analysis of integrin receptor subunits expression per tumor type. Black circles, malignant tumors; grey circles, benign tumors. Horizonal bars represent medians. ( B ) Representative image of β 3 integrin immunostaining on a mammary gland adenocarcinoma showing tumor cell and stromal staining (brown, β 3 integrin positive stain; cell nuclei are stained with Hematoxylin in blue). Scale bar, 100 microns.

Article Snippet: A 96-well microtiter plate was coated overnight at 4° C with 1 μg/mL recombinant human α v β 3 integrin (Merck-Millipore, Darmstadt, Germany) in coating buffer (20 mM Tris HCl pH 7.4; 150 mM NaCl; 1 mM MnCl 2 ; 0.5 mM MgCl 2 ; 2 mM CaCl 2 ).

Techniques: Western Blot, Expressing, Immunostaining, Staining

Journal: Oncotarget

Article Title: Preliminary safety and imaging efficacy of the near-infrared fluorescent contrast agent DA364 during fluorescence-guided surgery in dogs with spontaneous superficial tumors

doi: 10.18632/oncotarget.27633

Figure Lengend Snippet: Semi-quantitative (score) and quantitative (area) evaluation of anti-b 3 integrin staining in tumor samples

Article Snippet: A 96-well microtiter plate was coated overnight at 4° C with 1 μg/mL recombinant human α v β 3 integrin (Merck-Millipore, Darmstadt, Germany) in coating buffer (20 mM Tris HCl pH 7.4; 150 mM NaCl; 1 mM MnCl 2 ; 0.5 mM MgCl 2 ; 2 mM CaCl 2 ).

Techniques: Staining

Journal: Oncotarget

Article Title: CXCR6-CXCL16 axis promotes prostate cancer by mediating cytoskeleton rearrangement via Ezrin activation and α v β 3 integrin clustering

doi: 10.18632/oncotarget.6944

Figure Lengend Snippet: Panel-A. Expression of integrin α v β 3 in PCa cell lines. Untreated or CXCL16 treated PCa cells stained for integrin α v β 3 and nucleus stained with Draq5. Panel-B. Quantitative analysis of CXCL16 induced integrin α v β 3 clustering using ImageStream100 image based flow cytometer. Bright field (white), Integrin α v β 3 (green) and Draq5 (red), composite images for representative PC3 and LNCaP cells are shown. Panel-C. Percentage of cells showing α v β 3 integrin clustering in response to CXCL16 treatment with or without PKC/FAK inhibition. Integrin clustering was quantified using two standard image-based software analysis features: Area aspect ratio and Radial delta centroid, which demonstrated three types of cell population in samples: Uniform (global distribution of integrin α v β 3 on cell surface; cells with high aspect ratio with low radial delta centroid values), Capped (integrin α v β 3 clustered in a specific cell area; cells with high aspect ratio values and relatively low radial delta centroid) and Atypical (cell debris; cells with smaller aspect ratio and low radial delta centroid).

Article Snippet: Briefly, the cells were stained with 1 μg of FITC-conjugated mouse anti-human α v β 3 antibody (BD PharMingen) at 4°C for 30 min.

Techniques: Expressing, Staining, Flow Cytometry, Inhibition, Software

Journal: Biomedicines

Article Title: Triazole Modified Tetraiodothyroacetic Acid Conjugated to Polyethylene Glycol, a Thyrointegrin α v β 3 Antagonist as a Radio- and Chemo-Sensitizer in Pancreatic Cancer

doi: 10.3390/biomedicines10040795

Figure Lengend Snippet: Signaling pathways significantly affected by the P-bi-TAT treatment of human pancreatic carcinoma SUIT2 cells.

Article Snippet: Vibrant MTT cell proliferation assay kit was purchased from Life Technologies Corporation (Carlsbad, CA, USA), and fluorescein isothiocyanate (FITC) conjugated antibody of integrin α v β 3 was purchased from BD Biosciences (San Jose, CA, USA).

Techniques: Ligand Binding Assay, Activity Assay, Homologous Recombination